FIG. 1.

Lipid peroxidation products increased the expression of genes involved in the HB-EGF-activated EGFR pathway in the vascular endothelial cells. The human aortic endothelial cells isolated from 96 different human donors were treated with vehicle and oxidized phospholipid (Ox-PAPC, 50 μg/mL) for 4 hr. The HB-EGF, ADAM10, -17, and EGFR transcript values from the microarray dataset of the donor cells were plotted by order of basal transcript levels. The openly available dataset was reanalyzed (NCBI GEO: www.ncbi.nlm.nih.gov/geo/; reference number GSE20060).⁶³ The unique probe ID was inserted for each gene. P values mean the differential P-value from the Student's t-test for paired values of the vehicle and Ox-PAPC treatment groups after adjustment for multiple comparisons. ADAM, a disintegrin and metalloproteinase; EGFR, epidermal growth factor receptor; HB-EGF, heparin-binding EGF-like growth factor; Ox-PAPC, oxidized 1-palmitoyl-2-arachidonyl-sn-glycero phosphorylcholine.