Table 1.
In silico tools and databases used in the present study
| Tool | URL | Input |
|---|---|---|
| PolyPhen‐2 (investigation of variants) | http://genetics.bwh.harvard.edu/pph2 | Sequence |
| PopMusic (ΔΔG) | http://dezyme.com | Uploaded 3D structure |
| DUET (ΔΔG) | http://biosig.unimelb.edu.au/duet | Uploaded 3D structure |
| MaestroWeb (ΔΔG) | https://biwww.che.sbg.ac.at/maestro/web | Uploaded 3D structure |
| SAAFEC (ΔΔG) | http://compbio.clemson.edu/SAAFEC | Uploaded 3D structure |
| Missense3D | http://www.sbg.bio.ic.ac.uk/~missense3d | Protein sequence or 3D structure/PDB ID |
| VarSite | https://www.ebi.ac.uk/thornton-srv/databases/VarSite | UniProt ID, or search terms or disease |
| FlexPred (flexibility prediction) | http://kiharalab.org/flexPred | Uploaded 3D structure |
| PredyFlexy (flexibility prediction) | http://www.dsimb.inserm.fr/dsimb_tools/predyflexy | Sequence |
| Clustal Omega (multiple sequence alignment) | https://www.ebi.ac.uk/Tools/msa | Sequence |
| meta‐PPISP (prediction of PPI sites) | http://pipe.scs.fsu.edu/meta-ppisp | Uploaded 3D structure |
| FTMap (binding pocket prediction) | http://ftmap.bu.edu/home.php | Uploaded 3D structure |
| ClusPro (macromolecular docking) | https://cluspro.org | Uploaded 3D structure |
| PPM (protein membrane interaction prediction) | http://opm.phar.umich.edu | Uploaded 3D structure |
| pyDockWeb (macromolecular docking) | https://life.bsc.es/pid/pydock | Uploaded 3D structure |
| RING‐2.0 (residue interaction network) | http://protein.bio.unipd.it/ring | Uploaded 3D structure |
| Cytoscape (data visualization) | http://www.cytoscape.org | RING output XLM file |
| ChannelsDB (investigate channels) | https://webchemdev.ncbr.muni.cz/ChannelsDB | PDB ID |
| PharmVar (variation database) | https://www.pharmvar.org/htdocs/archive/index_original.htm | — |
| gnomAD (Genome Aggregation Database) | https://gnomad.broadinstitute.org/ | — |
| ClinVar (genomic variation database) | https://www.ncbi.nlm.nih.gov/clinvar/ | — |
| Chimera (molecular graphics) | http://www.cgl.ucsf.edu/chimera | — |
| PyMol (molecular graphics) | http://www.pymol.org | — |
| PDB (protein structure database) | https://www.rcsb.org | — |
| UniProt (annotated protein database) | http://www.uniprot.org | — |