Figure 3.

XIST is a ceRNA for miR‐93‐5p to promote the level of HIF‐1A. (a) The putative 3′UTR site for miR‐93‐5p binding of HIF‐1A mutation and wild‐type sequences (HIF‐1A‐MUT) and (HIF‐1A‐WT), respectively. (b, c) The mRNA and protein expressions of HIF‐1A in LoVo and SW480 cells. (d) Luciferase activity assay. (e) The miR‐93‐5p expression in LoVo cells transfected with NC or si‐XIST. Unpaired Student's t‐test was conducted to analyze data. (f) RIP test of the AGO2 enrichment of HIF‐1A and XIST transcripts compared with IgG of pLVX‐XIST or si‐XIST transfected cells. (g) The activity of luciferase of pGL3 reporters containing mutant HIF‐1A or wild‐type 3′UTR, which has indicated treatment in colorectal cancer cells. (h) The mRNA expression of HIF‐1A in SW480 and LoVo cells by qRT‐PCR analysis. *p < .05 versus. pLVX group, ^# p < .05 versus. XIST group in SW480 cells. ^# p < .05 versus. si‐XIST group in LoVo cells, *p < .05 versus. NC group. One‐way ANOVA was performed to analyze data