Figure 4.

XIST modulates colorectal cancer activity by in vitro AXL signaling. (a, b) The protein and mRNA levels of AXL in LoVo and SW480 cells. Unpaired Student's t‐test was performed to analyze data. (c) XIST stably transfected SW480 cells were treated with BGB324 for 72 hr at the concentration of 1, 0.5, and 0.1 nM to detect the cell vitality. One‐way ANOVA was performed to analyze data. (d) The protein contents of Vimentin, Snail, and E‐cadherin in SW480 cells with stable transfection of XIST, and the cells were disposed with BGB324 at various concentration and detected with western blot. (e, f) The levels of AXL and HIF‐1A mRNA in paired colorectal cancer and adjacent normal samples. Paired Student's t‐test was conducted to analyze data. ^# p < .05 versus. XIST treated group, *p < .05 versus. control group. ***p < .001