Figure 4:

Comparison of Wnt signaling inhibition mediated by freely delivered antibodies versus antibody-nanoparticle conjugates. (A) In cancer cells, ligand/receptor interactions activate downstream oncogenic signaling. Antibodies can reduce signaling by binding receptors to lock them in a ligand unresponsive state, and antibody-NP conjugates can enhance signaling blockade by engaging multiple receptors simultaneously. (B) Immunofluorescence analysis of β-catenin expression in MDA-MB-231 triple negative breast cancer cells shows that FZD7 antibody-nanoshell (FZD7-NS) conjugates are more effective than freely delivered antibodies at suppressing β-catenin expression and nuclear localization. Reproduced with permission from reference 73, Riley et al. Small. Copyright 2017 Wiley.