Table 2.

Primer sequences and PCR assay parameter of genes encoding cell cycle regulatory proteins of tea used in semi-quantitative RT-PCR assay

S. No	Gene name	NCBI Acc. No	TIPA locus name	Primer sequence (5′-3′)	Product size (bp)	No. of cycles	Annealing temperature (°C)
1	Histone H2A	HM003232	TEA025242.1	F: GGAGCTCCGGTCTACCTCGC	150	26	65
				R: TGAATGTTAGGCATCACTCCGCCA
2	Histone H2B.3	GW696866	TEA025244.1	F: GAGAAAGCTCCGGCGGAGAAGAA	300	24	63
				R: TCAAGAGCTAGTGAACTTAGTAACCGCC
3	Histone H3	HM003305	TEA032511.1	F: ATGGCTCGTACAAAGCAGACAGCTCG	350	24	63
				R: TCAAGCTCTCTCGCCTCGAATCCTCC
4	Histone H4	HM003230	NA	F: ATGTCGGGAAGAGGAAAGGGAGGAA	250	24	63
				R: CTAACCCCCAAACCCATAGAGAGTTCTC
5	Cyclin B	JF795471	TEA021407.1	F: TGATTGACTGGCTCATTGAGGTGC	450	28	60
				R: ACTTGGTATGCCTCTCGCTTGTCT
6	Cyclophilin	HM003242	TEA002681.1	F: ATGTTGAGATTGCCGGAAAACCTGCTG	325	23	66
				R: TCACAAAGGAAGTTCGCCGCTGTCA
7	Cyclin-D1	X83369	TEA017808.1	F: ATCCTCTGTTGTCAATCCCCAC	200	27	56
				R: AAGAGGATTCATCACTTGGCCT
8	Cyclin D3	AB247282	TEA014197.1	F: CAGCTCTCACTGCAATTCTTGCCA	300	27	65
				R: TGGGTTTTCAGTCCAAGCCTCCT
9	Cyclin dependent kinase A	AB247281	TEA024500.1	F: TGTGGATGTGTGGTCAGTGG	200	22	65
				R: ACAGTGGCCAGGTCCTTAGA
10	Elongation factor 2	JK341471	TEA022285.1	F: TCTGAGGGCAGCAACTTCAG	350	34	55
				R: CGGGGCATTATACTCCAGGG
11	Tubulin alpha-3	DQ444294	TEA008342.1	F: TTGCTTTTGGAGCGCTTGTC	350	26	55
				R: CGAGGGTATGGCACAAGGTT
12	Ubiquitin-conjugating enzyme E2 variant 1D	HM003308	TEA006425.1	F: GAGCACACTTCAGCCCATGGCA	500	28	65
				R: TGGGGGCCACATGACAGTCCTT
13	Ubiquitin-40S ribosomal protein S27	HM003234	TEA024638.1	F: ACCCTCGCCGATTACAACATCCA	300	27	65
				R: CCCCAGAACACTCAGAAACCACCA
14	Ubiquitin-40S ribosomal protein S27 protein	HM003307	TEA024638.1	F: TATGCCGGGGGAGAGAGACAGA	400	22	63
				R: AGTCAACAGGGTCTTCTCAAGTGCG
15	Cs5.8S rRNA	FJ004886	NA	F: GCCCTCAACCTAATGGCTTC	125	22	63
				R: GATATCTCGGCTCTCGCATC