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. 2020 Apr 23;11(5):339–351. doi: 10.1007/s13238-020-00710-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Structural basis for assembly of the terminase complex. (A) Schematic diagram of domain organization of pUL28 and pUL33. (B) Views of overall structure of the terminase complex. Domains, N terminus, C terminus and secondary structural elements of pUL28 and pUL33 are labeled. Color schemes for pUL28 and pUL33 are the same as Fig. 3A. pUL15 is colored in blue with 70% transparency. Residues 433–477 that are disordered in the structure are labeled as dots. (C) The “thread a needle” interaction mode between pUL33 and pUL28. A model shown at the right-bottom depicts the interaction mode. Insets illustrate two zinc-finger structures. (D) The “spool of wires” assembly mode of three N-terminal domains of the terminase complex. A model shown at the right-bottom depicts the assembly mode. (E) Double fixation of the ATPase by two sets of three-helix bundle from two strut domains. pUL28 and pUL15 are highlighted in magenta and black outlines. Secondary structural elements from pUL15 are labeled in red. (F) The interactions between two adjacent subunits of the hexameric terminase assembly. The packing domain shows tight contacts with the N-lasso, reinforce and strut domains from the neighboring subunit in the black inset. Color scheme is same as in Figs. 2A and 3A. (G) The proposed regulatory re-arrangement of the nuclease using two sets of “regulators” from pUL15 and pUL28. Left: the location of two “regulators” in the hexameric terminase assembly; Right: zoom-in view of two “regulators” within one terminase complex. The regulators from pUL28 and pUL15 are highlighted in magenta and black outlines

Structural basis for assembly of the terminase complex. (A) Schematic diagram of domain organization of pUL28 and pUL33. (B) Views of overall structure of the terminase complex. Domains, N terminus, C terminus and secondary structural elements of pUL28 and pUL33 are labeled. Color schemes for pUL28 and pUL33 are the same as Fig. 3A. pUL15 is colored in blue with 70% transparency. Residues 433–477 that are disordered in the structure are labeled as dots. (C) The “thread a needle” interaction mode between pUL33 and pUL28. A model shown at the right-bottom depicts the interaction mode. Insets illustrate two zinc-finger structures. (D) The “spool of wires” assembly mode of three N-terminal domains of the terminase complex. A model shown at the right-bottom depicts the assembly mode. (E) Double fixation of the ATPase by two sets of three-helix bundle from two strut domains. pUL28 and pUL15 are highlighted in magenta and black outlines. Secondary structural elements from pUL15 are labeled in red. (F) The interactions between two adjacent subunits of the hexameric terminase assembly. The packing domain shows tight contacts with the N-lasso, reinforce and strut domains from the neighboring subunit in the black inset. Color scheme is same as in Figs. 2A and 3A. (G) The proposed regulatory re-arrangement of the nuclease using two sets of “regulators” from pUL15 and pUL28. Left: the location of two “regulators” in the hexameric terminase assembly; Right: zoom-in view of two “regulators” within one terminase complex. The regulators from pUL28 and pUL15 are highlighted in magenta and black outlines