Primers for cloning |
|
Fuw-teto-CAS9/XCAS9/dCAS9-pgk-puro Gibson F |
TATCGATAAGCTTGATATCGAATTCTCAGGCACCGGGCTTGCGGG |
Fuw-teto-CAS9/XCAS9/dCAS9-pgk-puro Gibson R |
ATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGACAAGAAGTACAGCAT |
Fuw-teto-control-pgk-puro F |
AATTCGCCACCATGGATTACAAAGACGATGACGATAAGTAGA |
Fuw-teto-control-pgk-puro R |
CCGGTCTACTTATCGTCATCGTCTTTGTAATCCATGGTGGCG |
plenti-CAS9-pgk-puro Gibson F |
ACCGACTCTAGAGGATCCGCCACCATGGACAAGAAGTACAGCATCGGCC |
plenti-CAS9-pgk-puro Gibson R |
TCCAGAGGTTGATTGTCGACCTACTTATCGTCATCGTCTT |
Fuw-teto-95-pgk-puro Gibson F |
GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGACAGCTTCTTCCACAGACTG |
Fuw-teto-95-pgk-puro Gibson R |
TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
plenti-719-pgk-puro Gibson F |
GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGAGCCTGCACGAGCACATT |
Fuw-teto-719-pgk-puro Gibson R |
TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
Fuw-teto-910-pgk-puro Gibson F |
GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGAACTGGATAAGGCCGGCTT |
Fuw-teto-910-pgk-puro Gibson R |
TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
Fuw-teto-1100-pgk-puro Gibson F |
GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGTGCAGACAGGCGGCTTCA |
Fuw-teto-1100-pgk-puro Gibson R |
TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
plenti-control-pgk-puro F |
GATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTTAGG |
plenti-control-pgk-puro R |
TCGACCTAAGCGTAGTCTGGGACGTCGTATGGGTACATGGTGGCG |
plenti-Ku86-pgk-puro Gibson F |
GACACCGACTCTAGAGGATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTATGGTGCGGTCGGGGAATAAG |
plenti-Ku86-pgk-puro Gibson R |
TCACAAATTTTGTAATCCAGAGGTTGATTGTCGACGAATTCCTATATCATGTCCAATAAATCGTCC |
Fuw-teto-Cpf1-pgk-puro Gibson F |
AAGCCCTCGAACTGTGTCATGGTGGCGAATTCGGGGCCGCGGAGGCTGGAT |
Fuw-teto-Cpf1-pgk-puro Gibson R |
AGACGATGACGATAAGTAGACCGGTTTGGGGTTGCGCCTTTTCCA |
Primers for RT-qPCR |
|
Puma F |
ACGACCTCAACGCACAGTACGA |
Puma R |
CCTAATTGGGCTCCATCTCGGG |
P21 F |
ACCTGGAGACTCTCAGGGTCG |
P21 R |
TTAGGGCTTCCTCTTGGAGAAGAT |
Perp F |
TCATCCTGTGCATCTGCTTC |
Perp R |
GGGTTATCGTGAAGCCTGAA |
Noxa F |
ACCAAGCCGGATTTGCGATT |
Noxa R |
ACTTGCACTTGTTCCTCGTGG |
β-actin F |
AGCGAGCATCCCCCAAAGTT |
β-actin R |
GGGCACGAAGGCTCATCATT |