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. 2020 Mar 13;11(5):352–365. doi: 10.1007/s13238-020-00699-6

Table 1.

Primers used in this study.

Sequence (5′-3′)
Primers for cloning
 Fuw-teto-CAS9/XCAS9/dCAS9-pgk-puro Gibson F TATCGATAAGCTTGATATCGAATTCTCAGGCACCGGGCTTGCGGG
 Fuw-teto-CAS9/XCAS9/dCAS9-pgk-puro Gibson R ATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGACAAGAAGTACAGCAT
 Fuw-teto-control-pgk-puro F AATTCGCCACCATGGATTACAAAGACGATGACGATAAGTAGA
 Fuw-teto-control-pgk-puro R CCGGTCTACTTATCGTCATCGTCTTTGTAATCCATGGTGGCG
 plenti-CAS9-pgk-puro Gibson F ACCGACTCTAGAGGATCCGCCACCATGGACAAGAAGTACAGCATCGGCC
 plenti-CAS9-pgk-puro Gibson R TCCAGAGGTTGATTGTCGACCTACTTATCGTCATCGTCTT
 Fuw-teto-95-pgk-puro Gibson F GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGACAGCTTCTTCCACAGACTG
 Fuw-teto-95-pgk-puro Gibson R TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC
 plenti-719-pgk-puro Gibson F GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGAGCCTGCACGAGCACATT
 Fuw-teto-719-pgk-puro Gibson R TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC
 Fuw-teto-910-pgk-puro Gibson F GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGAACTGGATAAGGCCGGCTT
 Fuw-teto-910-pgk-puro Gibson R TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC
 Fuw-teto-1100-pgk-puro Gibson F GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGTGCAGACAGGCGGCTTCA
 Fuw-teto-1100-pgk-puro Gibson R TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC
 plenti-control-pgk-puro F GATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTTAGG
 plenti-control-pgk-puro R TCGACCTAAGCGTAGTCTGGGACGTCGTATGGGTACATGGTGGCG
 plenti-Ku86-pgk-puro Gibson F GACACCGACTCTAGAGGATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTATGGTGCGGTCGGGGAATAAG
 plenti-Ku86-pgk-puro Gibson R TCACAAATTTTGTAATCCAGAGGTTGATTGTCGACGAATTCCTATATCATGTCCAATAAATCGTCC
 Fuw-teto-Cpf1-pgk-puro Gibson F AAGCCCTCGAACTGTGTCATGGTGGCGAATTCGGGGCCGCGGAGGCTGGAT
 Fuw-teto-Cpf1-pgk-puro Gibson R AGACGATGACGATAAGTAGACCGGTTTGGGGTTGCGCCTTTTCCA
Primers for RT-qPCR
 Puma F ACGACCTCAACGCACAGTACGA
 Puma R CCTAATTGGGCTCCATCTCGGG
 P21 F ACCTGGAGACTCTCAGGGTCG
 P21 R TTAGGGCTTCCTCTTGGAGAAGAT
 Perp F TCATCCTGTGCATCTGCTTC
 Perp R GGGTTATCGTGAAGCCTGAA
 Noxa F ACCAAGCCGGATTTGCGATT
 Noxa R ACTTGCACTTGTTCCTCGTGG
 β-actin F AGCGAGCATCCCCCAAAGTT
 β-actin R GGGCACGAAGGCTCATCATT