Table 1.
Primers used in this study.
| Sequence (5′-3′) | |
|---|---|
| Primers for cloning | |
| Fuw-teto-CAS9/XCAS9/dCAS9-pgk-puro Gibson F | TATCGATAAGCTTGATATCGAATTCTCAGGCACCGGGCTTGCGGG |
| Fuw-teto-CAS9/XCAS9/dCAS9-pgk-puro Gibson R | ATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGACAAGAAGTACAGCAT |
| Fuw-teto-control-pgk-puro F | AATTCGCCACCATGGATTACAAAGACGATGACGATAAGTAGA |
| Fuw-teto-control-pgk-puro R | CCGGTCTACTTATCGTCATCGTCTTTGTAATCCATGGTGGCG |
| plenti-CAS9-pgk-puro Gibson F | ACCGACTCTAGAGGATCCGCCACCATGGACAAGAAGTACAGCATCGGCC |
| plenti-CAS9-pgk-puro Gibson R | TCCAGAGGTTGATTGTCGACCTACTTATCGTCATCGTCTT |
| Fuw-teto-95-pgk-puro Gibson F | GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGACAGCTTCTTCCACAGACTG |
| Fuw-teto-95-pgk-puro Gibson R | TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
| plenti-719-pgk-puro Gibson F | GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGAGCCTGCACGAGCACATT |
| Fuw-teto-719-pgk-puro Gibson R | TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
| Fuw-teto-910-pgk-puro Gibson F | GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGAACTGGATAAGGCCGGCTT |
| Fuw-teto-910-pgk-puro Gibson R | TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
| Fuw-teto-1100-pgk-puro Gibson F | GACCGATCCAGCCTCCGCGGCCCCGAATTCGCCACCATGGTGCAGACAGGCGGCTTCA |
| Fuw-teto-1100-pgk-puro Gibson R | TGGAAAAGGCGCAACCCCAAACCGGTCTACTTATCGTCATCGTCTTTGTAATC |
| plenti-control-pgk-puro F | GATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTTAGG |
| plenti-control-pgk-puro R | TCGACCTAAGCGTAGTCTGGGACGTCGTATGGGTACATGGTGGCG |
| plenti-Ku86-pgk-puro Gibson F | GACACCGACTCTAGAGGATCCGCCACCATGTACCCATACGACGTCCCAGACTACGCTATGGTGCGGTCGGGGAATAAG |
| plenti-Ku86-pgk-puro Gibson R | TCACAAATTTTGTAATCCAGAGGTTGATTGTCGACGAATTCCTATATCATGTCCAATAAATCGTCC |
| Fuw-teto-Cpf1-pgk-puro Gibson F | AAGCCCTCGAACTGTGTCATGGTGGCGAATTCGGGGCCGCGGAGGCTGGAT |
| Fuw-teto-Cpf1-pgk-puro Gibson R | AGACGATGACGATAAGTAGACCGGTTTGGGGTTGCGCCTTTTCCA |
| Primers for RT-qPCR | |
| Puma F | ACGACCTCAACGCACAGTACGA |
| Puma R | CCTAATTGGGCTCCATCTCGGG |
| P21 F | ACCTGGAGACTCTCAGGGTCG |
| P21 R | TTAGGGCTTCCTCTTGGAGAAGAT |
| Perp F | TCATCCTGTGCATCTGCTTC |
| Perp R | GGGTTATCGTGAAGCCTGAA |
| Noxa F | ACCAAGCCGGATTTGCGATT |
| Noxa R | ACTTGCACTTGTTCCTCGTGG |
| β-actin F | AGCGAGCATCCCCCAAAGTT |
| β-actin R | GGGCACGAAGGCTCATCATT |