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. 2020 Apr 27;11:607. doi: 10.3389/fimmu.2020.00607

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Copyright © 2020 Cai, Caraballo Galva, Peng, Luo, Zhu, Yao, Ji and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The EC₅₀ and EC₉₀ of AFP₁₅₈ peptide for activating TCR-Ts. (A) TCR-Ts were generated by transducing primary human T cells with TCR genes. Representative dot plots showed the% of TCR + T cells 12–15 days after transduction. (B,C) The EC₅₀ of AFP158 peptide for activating TCR-Ts were presented. The EC₉₀ was calculated using Prism software. (D) The EC_XX value corresponding to the 10 ng/ml of AFP₁₅₈ peptide for activating different TCR-Ts was calculated using Prism software. The experiments were repeated three times with similar data.