FIGURE 2.

Identification of peptide motifs that can be recognized by TCR-Ts. (A) The scheme of X-Peptides. “X” in each position represents any of the other 19 amino acids. (B) A representative data (position 1–3 of TCR1-T) of X-Scan assay showed the response ratio of X-peptide vs. AFP₁₅₈ peptide in stimulating TCR-Ts to produce IFNγ. The Mean ± SD from 3 wells was shown. The X-scan assay for each peptide was repeated 3–5 times and consistent observation was obtained. (C) X-Scan identified the peptide motifs potentially recognized by TCR-Ts. The colored letters indicate the difference among 3 TCR-Ts. A common motif that cover all 3 TCR-Ts is also shown. (D) Comparison of A-, G-, and X-Scan to reveal the number of the tolerant amino acids at each position AFP₁₅₈ epitope.