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Synthetic off-target peptides cross-activate TCR-Ts. (A) The activation of TCR-Ts to produce IFNγ by 5 peptides at high (1 μg/ml) and low (0.01 μg/ml) concentrations is presented. The data shown was the response ratios of off-target peptides vs. AFP158. (B) Shown is the dose-dependent production of IFNγ by TCR-Ts after stimulation with different peptides. ANOVA was used for statistical analysis. (C,D) The EC50 of AFP158 and ENPP1436 was measured and compared.
