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Tumor cells overexpressing ENPP1 do not activate TCRTs. (A) Western Blot analysis showed the ectopic overexpression of ENPP1 in MCF7 cells after transient gene transfer. (B) LDH assay showed that TCR-Ts did not kill the MCF7 cells that overexpress ENPP1 protein. (C) ELISA assay indicated that overexpression of ENPP1 in MCF7 did not activate TCR-Ts to produce IFNγ.
