Figure 7.

C₁₄PC protects primary human monocytes from lysis by both PVL and α-toxin. A, protective effect of C₁₄PC against PVL-mediated killing of monocytes. PBMCs were challenged with PVL in the presence and absence of 100 μm C₁₄PC. Monocyte subsets were gated based on the forward scatter and side scatter parameters and then analyzed for the expression of CD14⁺ and CD3⁺, respectively. Cell viability was determined by flow cytometry and normalized to that in the medium control. Data are representative of two independent experiments using blood from different donors. Percentages of cells in each quadrant are indicated. B, bar graph showing inhibition of the PVL-induced lysis of monocytes by 100 μm C₁₄PC. Error bars, S.E. C, protective effect of C₁₄PC against α-toxin–mediated killing of monocytes. PBMCs were challenged with α-toxin in the presence and absence of 100 μm C₁₄PC. Monocyte subsets were identified and quantified as described in A. Data are representative of two independent experiments using blood from different donors. D, bar graph showing inhibition of the α-toxin–induced lysis of monocytes by 100 μm C₁₄PC. Error bars, S.E.