Table 2.
kcat/Km values (×10−3m−1s−1) of wildtype SHP2 and T507K mutant with pTyr-peptides as substrates
| Enzyme | Substrate |
|||
|---|---|---|---|---|
| Sprouty1/pY53 | Paxillin/pY118 | Gab1/pY589 | EGFR/pY992 | |
| WT(CD) | 110 ± 0.9 | 123 ± 1.0 | 60 ± 0.7 | 1167 ± 23 |
| T507K(CD) | 50.0 ± 0.8 | 10.5 ± 0.2 | 11.0 ± 0.3 | 72.5 ± 1.5 |
| WT(FL) | 5.1 ± 0.1 | 5.2 ± 0.2 | 3.0 ± 0.07 | 95 ± 1.4 |
| T507K(FL) | 8.5 ± 0.1 | 0.8 ± 0.01 | 0.6 ± 0.01 | 15.0 ± 0.5 |
| Change in kcat/Km | ||||
| WT(CD)/T507K(CD) | 2.2 | 11.7 | 5.5 | 16.1 |
| WT(FL)/T507K(FL) | 0.6 | 6.5 | 5.0 | 6.3 |
| Extent of autoinhibition | ||||
| WT (CD/FL)kcat/Km | 21.6 | 23.7 | 20.0 | 12.3 |
| T507K (CD/FL)kcat/Km | 5.9 | 13.1 | 18.3 | 4.8 |