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. 2020 Mar 17;295(18):5970–5983. doi: 10.1074/jbc.RA119.011049

Figure 1.

Figure 1.

DHHC7 is an important barttin palmitoyl acyltransferase. A, HEK293T cells co-expressing barttin and the indicated DHHCs were analyzed by ABE. Representative blots are shown on the left, and quantifications of barttin palmitoylation after co-expression of DHHC3, DHHC6, DHHC7, and DHHC20 (n = 3) are shown on the right (see also Fig. S1). **, p < 0.01, one-way ANOVA; Dunnett's multiple-comparison test. B and D, bar graphs indicate the expression level of DHHC7 mRNA (n = 4; B) or DHHC3 mRNA (n = 6; D) in HEK293T cells after transfection with shRNA against DHHC7 or DHHC3, respectively. RT-qPCR analysis was evaluated by the ΔΔCt method. ***, p < 0.001, one-way ANOVA, Tukey's multiple-comparison test. C and E, ABE analysis of barttin palmitoylation in HEK293T cells after knockdown of endogenous DHHC7 (C) or DHHC3 (E) by shRNA (left panels). Quantification is shown on the right (see also Fig. S2A). Western blots are representative of at least three independent experiments. **, p < 0.01, Student's t test (for C); one-way ANOVA, Dunnett's multiple-comparison test (for E). All data are shown as mean + S.D. (error bars).