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Disease-causing barttin mutation R8L affects palmitoylation of barttin. A, representative image from ABE palmitoylation assay followed by SDS-PAGE and Western blotting for WT and R8L barttin mutant. B, relative palmitoylation levels of WT and R8L barttin mutant. Data are shown as means + S.D. (error bars) (n = 4). *, p < 0.05. Student's t test. C, putative topology of barttin with positions of Arg-8, Cys-54, and Cys-56.
