Figure 8.

NF-κB regulates MyD88 pre-mRNA splicing. A and B, RAW264.7 cells stably overexpressing the MyD88 minigene plasmid were pulse-treated with 200 ng/ml LPS for 2 h (or were left untreated as a control) in either the presence or absence of the IKK2 inhibitor TPCA1. After a further 22-h incubation with or without TPCA1, RNA was collected, and MyD88-L(minigene) and MyD88-S(minigene) mRNA levels were assessed by qPCR. These inhibitor studies were performed in the presence of two apoptosis inhibitors, Z-VAD-fmk and necrostatin (n = 3). Data are normalized so that MyD88-L(mini) or MyD88-S(mini) expression in the absence of LPS is set to 1. *, p < 0.05.