Figure 7.

Interfering with basal autophagic flux leads to an accumulation of intracellular VEGFA. A, representative immunoblots of PAER2 treated with increasing concentrations of bafilomycin A1 (BafA1) for 6 h. B, quantification of targets as in A. C and D, immunoblot of HUVEC ± BafA1 (500 nm) (C) and quantification (D). E and F, immunoblot of HUVEC ± chloroquine (30 μm) for 6 h (E) and quantification (F). G and H, immunoblot depicting HUVEC following the transient transfection of siScr or siATG5 (100 pm each) (G) and corresponding quantification of targets (H) as in G. GAPDH served as the loading control for immunoblots depicted in A, C, E, and G. Data are reflective of four independent biological replicates. Data are expressed as arbitrary units (A.U.) on a dot density plot. Statistics were calculated via two-tailed Student's t test for D, F, and H.