Figure 2.

Zinc-induced tau441 droplets are liquid-like. A, representative fusion events between tau441 (10 μm) droplets induced by 40 μm zinc (Zn²⁺/tau441 molar ratio of 4:1) in low-ionic strength buffer (10 mm HEPES, 10 mm NaCl, pH 7.4) without any crowding agent. Scale bar, 2 μm. B, FRAP traces for tau441 droplets in low-ionic strength buffer (10 mm NaCl, no crowding agent) obtained 10 min (red) and 120 min (green) after inducing LLPS with 40 μm Zn²⁺. C, representative fusion events between tau441 (10 μm) droplets induced by 40 μm zinc (Zn²⁺/tau441 molar ratio of 4:1) in a buffer containing 100 mm NaCl and 10% PEG 3. Scale bar, 2 μm. D, FRAP traces for tau droplets in a buffer containing 100 mm NaCl and 10% PEG 3 obtained 10 min (red) and 120 min (green) after inducing LLPS with 40 μm Zn²⁺. Inset, images of droplets at 0 and 125 s after photobleaching (scale bar, 1 μm). Error bars, S.D. (n = 4).