Figure 5.

Prom3 influences molecular left-right asymmetry. A–E, embryos were injected at the one-cell stage with either one of the three MOs against prom3 (A–D, MO1 (2.5 ng); E, MO1 (2.5 and 5 ng), MO2 (5 and 10 ng), and MO3 (10 ng)) or control MO (E, 10 ng). At 25 hpf, specimens were processed for whole-mount ISH using antisense DIG-labeled lefty-2 probe (A–D). Note the randomized expression of lefty-2 (left, right, and bilateral) with regard to the median-sagittal plane (dashed line) or its absence. Shown are frequencies of the lefty-2 position (see color code) observed in prom3-morphants upon application of a given MO at various amounts (E). The number of embryos analyzed is indicated in parentheses. Orientation is as follows. A–D, dorsal (rostral toward the top).