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. 2020 Mar 22;295(18):6007–6022. doi: 10.1074/jbc.RA119.011253

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© 2020 Jászai et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — Prom3 regulates the number and length of cilia in the Kupffer's vesicle. A–D, embryos were injected at the one-cell stage with control (A, C, and D, ctrl, 10 ng) and MO against prom3 (B, C, and D, MO2; C and D, MO3, 10 ng) and processed at the 8-somite stage for double immunofluorescence using anti-AcTub (red) and anti-aPKCζ (green) antibodies, counterstained with DAPI (blue), and analyzed by CLSM. The number (C) and length (D) of cilia in the Kupffer's vesicle (KV) were quantified. The total numbers of Kupffer's vesicles and cilia analyzed are indicated in parentheses. The mean and S.D. (error bars) are presented. Individual values are shown. *, p < 0.05; ***, p < 0.001. Scale bars (A and B), 10 μm.