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. 2020 Mar 30;295(18):6120–6137. doi: 10.1074/jbc.RA119.011138

Figure 2.

Figure 2.

Chronic 6-OH–BDE-47 exposure impairs evoked synaptic activity. Multiple stimulus paradigms were used to assess the effects of chronic 6-OH exposure on evoked electrical activity. A, graphical depiction of bicuculline (Bic) stimulus paradigms. B, example of characteristic burst activity generated with Bic stimulus. C and D, quantification of spikes and bursts detected under continual Bic treatment of neurons chronically exposed to 500 nm 6-OH (from time of plating to day of experiment), n = 5–8. p values were generated with two-way ANOVA with post hoc LSD. Spike data: time F(2,30) = 0.269, p = 0.76; treatment F(1,30) = 10.15, p = 0.0034; interaction F(2,30) = 0.235, p = 0.7920. Burst data: time F(2,30) = 2.356, p = 0.11; treatment F(1,30) = 12, p = 0.0016; interaction F(2,30) = 0.04056, p = 0.9603. E and F, same experimental design and presentation as in B, but after brief Bic stimulation and washout instead of continual treatment, n = 3–8. Spike data: time F(2,22) = 4.62, p = 0.021; treatment F(1,22) = 24.31, p < 0.0001; interaction F(2,22) = 2.8, p = 0.0825. Burst data: time F(2,22) = 1.57, p = 0.23; treatment F(1,22) = 25.03, p < 0.0001; interaction F(2,22) = 1.393, p = 0.2695.