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. 2020 Mar 25;295(18):6092–6107. doi: 10.1074/jbc.RA120.012829

Figure 3.

Figure 3.

Reverse transcription by hpol η. PAGE (20%, 7 m urea) was conducted. A and C, hpol η (200 nm) elongated DNA primer opposite rA-containing (A) and ϵrA-containing (C) RNA templates in the presence of a mixture of dNTPs or rNTPs (physiological concentrations). All reactions were done at 37 °C for 5, 30, 60, and 180 min (time gradients indicated with wedges). Lanes 1–4, dNTPs; lanes 5–8, rNTPs. B and D, hpol η (50 nm) was incubated with DNA/RNA-rA; DNA/RNA-1,N6-ϵrA with individual dNTPs and rNTPs (physiological concentrations). Lanes 1–3, dTTP; lanes 4–6, dATP; lanes 7–9, dCTP; lanes 10–12 dGTP; lanes 13–15, rUTP; lanes 16–18, rATP; lanes 19–21, rCTP; lanes 22–24, rGTP. All reactions were done at 37 °C for 5, 30, and 60 min. See “Experimental procedures” and Table S1 for the oligonucleotide sequences used. P, FAM-labeled DNA primer.