Figure 7.

Human RNase H2–mediated incision of ribonucleotide embedded in DNA. PAGE (20%, 7 m urea) was conducted of incision assays employing human RNase H2 (10 nm) with dsDNAs and different pairing conditions. A, DNA-X/DNA-dT; B, DNA-X/DNA-dA; C, DNA-X/DNA-dG (where X represents dA, rA, or 1,N⁶-ϵrA). All reactions were done in duplicate at 37 °C for 5, 15, 30, 45, and 60 min (time gradients indicated with wedges). S, substrate position (23 nt); P, product position (4 nt). See “Experimental procedures” and Table S1 for the DNA sequences used (FAM-labeled templates and biotin-labeled complementary strands (CS) were used).