Figure 1.

In vitro dissociation of the 100S ribosome by the RRF/EF-G pair and HflX in the presence and absence of guanosine analogs. Reactions were programmed with 0.2 μm ribosomes, 2 μm proteins, and 2 mm GTP analogs and incubated at 37 °C for 30 min. The samples were centrifuged in a 5–20% sucrose gradient, and ribosome profiles were monitored from the absorbance at 254 nm (y axis). Quantification of the 100S to 70S ratios were obtained from three technical replicates (of two independently prepared ribosomes and recombinant proteins); mean ± S.D.