Table 2.
Mean (min.‐max.) concentration of BA derivates in different body fluids of heifers collected during corresponding OPU sessions
| Serum (ng/mL) | Follicular Fluid (ng/mL) | Urine (ng BA/ mg Cr) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Pro‐oestrus | Oestrus | Post‐oestrus | Interoestrus | Pro‐oestrus | Oestrus | Post‐oestrus | Interoestrus | Pro‐oestrus | Oestrus | Post‐oestrus | Interoestrus | |
| Cholic acid (CA) derivatives, mean % of total | 75.4% | 71.5% | 72.6% | 77.2% | 70.7% | 71.3% | 69.6% | 73.0% | ||||
| Cholic acid (CA) | 3,478.7 (154.6–4979.4) | 2,350.5 (446.9–4771.5) | 2,499.8 (368.3–7566.1) | 3,878.6 (327.7–13743.9) | 1738.0 (204.1–4069.7) | 1,890.5 (568.9–4317.1) | 1544.7 (494.2–2926.0) | 2,136.5 (120.5–6745.1) | 1,259.7 (76.0–3017.8) | 1617.3 (104.1–4141.4) | 644.0 (82.6–1495.4) | 1858.9 (79.4–6447.7) |
| Glycocholic acid (GCA) | 1,404.5 (206.5–2926.6) | 1,191.5 (390.9–3105.0) | 2010.2 (533.2–4672.8) | 1874.9 (51.0–5535.8) | 1,105.1 (499.3–1785.5) | 1,324.3 (364.8–3255.4) | 1758.9 (510.9–3803.4) | 1,488.4 (114.1–4763.4) | 126.0 (84.2–178.3) | 135.0 (77.5–236.1) | 141.8 (66.6–293.3) | 172.8 (70.2–447.0) |
| Taurocholic acid (TCA) | 619.9 (98.0–1520.8) | 583.4 (151.9–933.4) | 776.5 (209.6–2367.9) | 615.8 (36.7–1925.1) | 522.4 (114.1–1568.7) | 646.3 (182.8–1742.2) | 744.6 (129.4–1933.3) | 581.4 (17.5–1901.9) | 50.6 (4.0–102.5) | 56.0 (34.9–97.3) | 51.4 (28.5–89.6) | 54.3 (13.0–144.2) |
| Chenodeoxycholic acids (CDCA) derivatives, mean % of total | 8.1% | 8.6% | 9.8% | 8.0% | 10.3% | 9.1% | 10.2% | 9.2% | ||||
| Chenodeoxycholic acids (CDCA) | 91.5 (97–201.1) | 63.5 (14.4–187.7) | 50.1 (12.0–119.3) | 88.8 (10.5–332.3) | 54.8 (9.8–118.3) | 68.4 (8.2–261.0) | 50.6 (17.5–163.5) | 58.2 (5.5–306.6) | n.d. | n.d. | n.d. | n.d. |
| Glycochenodeoxycholic acid (GCDCA) | 324.9 (27.8–722.5) | 263.9 (14.7–830.2) | 461.8 (180.5–681.7) | 367.7 (19.3–854.6) | 259.5 (78.9–475.5) | 255.5 (64.1–644.3) | 331.3 (115.9–652.7) | 280.6 (7.0–489.8) | n.d. | n.d. | n.d. | n.d. |
| Taurochenodeoxycholic acid (TCDCA) | 178.5 (13.9–603.9) | 167.5 (34.5–352.1) | 201.9 (30.6–474.6) | 205.1 (7.7–494.1) | 176.4 (33.4–548.7) | 169.2 (21.0–429.4) | 213.0 (17.7–357.9) | 189.2 (10.5–588.4) | n.d. | n.d. | n.d. | n.d. |
| Deoxycholic acid (DCA) derivatives, mean % of total | 16.5% | 19.9% | 17.4% | 14.8% | 19.0% | 19.6% | 20.1% | 17.9% | ||||
| Deoxycholic acid (DCA) | 259.6 (4.0–584.1) | 268.9 (36.4–598.8) | 160.7 (19.1–417.3) | 230.6 (21.3–744.3) | 122.2 (14.3–245.2) | 159.1 (35.2–394.7) | 101.3 (21.2–247.1) | 129.1 (11.1–390.7) | 37.7 (5.8–81.2) | 38.6 (11.9–62.3) | 24.2 (7.5–44.6) | 37.5 (7.8–128.8) |
| Glycodeoxycholic acid (GDCA) | 536.7 (102.6–1018.1) | 514.5 (211.7–1447.5) | 618.8 (295.0–1010.8) | 568.9 (24.9–1286.9) | 426.6 (143.1–767.3) | 476.7 (183.7–950.8) | 558.5 (209.8–976.8) | 472.8 (47.8–982.2) | 10.4 (7.1–16.5) | 17.2 (7.4–30.1) | 16.5 (8.0–35.3) | 14.0 (5.4–35.9) |
| Taurodeoxycholic acid (TDCA) | 407.2 (55.4–1205.6) | 365.4 (85.8–658.9) | 486.7 (179.6–1155.2) | 425.1 (78.0–946.2) | 355.3 (71.6–1073.2) | 425.0 (51.81092.2) | 511.1 (69.6–939.9) | 429.1 (10.5–1215.8) | n.d. | n.d. | n.d. | n.d. |
Abbreviation: n.d., not detectable.