Fig. 2.

Transcription assay in the presence of Rad26 and TFIIS. Pol II ECs were prepared with a full-bubble scaffold, and transcription assay was performed in the presence or absence of 100 nM Rad26 and 300 nM TFIIS. Time points are 0 (control, without rNTPs), 1 min, 3 min, 10 min, and 30 min. Rad26 was added before the reaction started, while TFIIS was added together with 1 mM of rNTPs because preincubation with TFIIS and EC leads to partial degradation of RNA. Addition of Rad26 or TFIIS did not change the stalling patterns of the Gh or Sp lesion-containing scaffold.