Fig. 4.

Transcriptional pausing or bypass of consecutive oxidative lesions. (A) Sequences of scaffold used in transcription extension assay. X represents G, 8OG, Gh, R-Sp, or S-Sp, respectively. Y represents A, C, or G, respectively. The color code is the same as Fig. 1B. (B–E) Extension (Upper) assay beyond the oxidative lesion using 10C (Fig. 4 B and C), 10A (Fig. 4 D and E), and 10G (SI Appendix, Fig. S4) primer, respectively. The reaction was incubated with either a mixture of four rNTPs (1 mM) (Fig. 4 B and D and SI Appendix, Fig. S4) or individual rNTPs as shown in the figure panels (Fig. 4 C and E and SI Appendix, Fig. S4). The time points are 0, 10 s, 3 min, 30 min, and 2 h.