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ANI regulates the levels of inflammatory cytokines by inhibiting NLRP3 expression. The mice were randomly divided into four groups (n = 5): Control group, ANI-200 group, ANI-200+pcDNA-NLRP3 group and ANI-200+EV group. (A–D) The levels of INF-γ, TNF-α, IL-4 and IL-27 were measured by ELISA. (E–F). Th1 and Th2 cell subsets were sorted by flow cytometry. (*p < 0.05 vs control group, #p < 0.05 vs ANI-200 group, &p < 0.05 vs ANI-200+pcDNA-NLRP3 group).
