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. 2020 Feb 17;39(9):e103788. doi: 10.15252/embj.2019103788

Figure 1. In vitro assembly and cryo‐EM structure of the archaeal post‐splitting complex.

Figure 1

  1. ABCE1IIEA efficiently splits 70S ribosomes in the presence of AMP‐PNP and aRF1/aPelota. The 30S population contains a stoichiometric ratio of ABCE1 and ribosomal proteins, forming the post‐splitting complex. rps: small subunit ribosomal proteins.
  2. Cryo‐EM density of the post‐SC highlights the archaeal ribosomal protein eS21 and ABCE1. Domain architecture of ABCE1 including the mutation sites is shown below.
  3. Molecular model of the archaeal post‐SC, domain colors as in (B).
Data information: In (A), the gradient profiles are representative for the respective nucleotide condition.