Figure EV4. Detailed in vitro biochemical characterization of ABCE1 variants.

1. As wild‐type ABCE1, all variants are unable to bind 30S ribosomes in the presence of ADP or in the absence of nucleotide (no nt), thereby excluding that the respective mutation does not lead to unspecific binding to the ribosome.
2. Examples of sucrose density gradient profiles of 70S splitting reactions illustrate reduced splitting efficiencies of hinge 2 mutants compared to wild‐type ABCE1. SDG profile of the background control (aRF1/aPelota) is similar to R565E, highlighting its essential anchoring function (see Fig 3C).

Source data are available online for this figure.