Table 1.
Data collection, refinement, and validation statistics
| 30S‐ABCE1 (EMD‐10519, PDB ID 6TMF) | |
|---|---|
| Data collection | |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 25 |
| Defocus range (μm) | −1.1 to −2.3 |
| Pixel size (Å) | 1.084 |
| Symmetry imposed | C1 |
| Refinement | |
| Particle images (no.) | 293 010 |
| Map resolution (Å) (overall/30S head/ABCE1) | 2.8/2.8/3.0 |
| FSC threshold | 0.143 |
| Map sharpening B factor (Å2) (overall/30S head/ABCE1) | −117.8/−128.8/−151.9 |
| Model composition | |
| Correlation coefficient (%; Phenix) | 0.85 |
| Initial model used (PDB codes) | 5JBH, ABCE1: 5LL6 (chain h) |
| Non‐hydrogen atoms | 65 449 |
| Protein residues | 4 172 |
| RNA bases | 1 485 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.017 (30) |
| Bond angles (°) | 1.208 (44) |
| Validation | |
| MolProbity score | 1.79 |
| Clash score | 4.66 |
| Rotamer outliers (%) | 0.73 |
| Ramachandran plot | |
| Favored (%) | 89.98 |
| Allowed (%) | 9.51 |
| Disallowed (%) | 0.51 |
| Validation RNA | |
| Correct sugar pucker (%) | 98 |
| Good backbone conf. (%) | 80 |