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. 2020 Apr 10;117(17):9311–9317. doi: 10.1073/pnas.1920880117

Fig. 2.

Fig. 2.

ZNF263 represses HS3ST1 and HS3ST3A1 expression. (A) TF-binding motifs (rows) significantly enriched in the set of HS biosynthesis genes (columns) based on cis-eQTL analysis. (B) CRISPR sgRNA targeting of human ZNF263 in HeLa cells. Frameshift mutations in the KRAB domain were confirmed in clone 2B and clone 5 (SI Appendix, Fig. S2A). (C) Fold change in mRNA expression of ZNF263 target genes in ZNF263−/− cells relative to wild-type cells. Only genes containing a ZNF263-binding motif are shown (Fig. 1C) (n = 4; t test after log2 transformation; Sidak–Holm correction for multiple tests). (D) mRNA expression of HS3ST1 and HS3ST3A1 in various primary, cancer, and CHO cell lines was measured after siRNA-mediated knockdown of ZNF263 mRNA (n = 4; t test after log2 transformation; Sidak–Holm correction for multiple tests). n.d., not detected.