Figure 4. Identification of Cbf1 as a ncRNA‐binding protein.

• A
  Representative gel images of pull‐downs performed with ncRNAs as baits (compare to Fig 3B). Cbf1 was identified as a specific binder of all csRNAs and F41, but not of riboswitch RNA F20.
• B
  Heat map showing Spearman's correlation coefficients between the sedimentation profiles of bait ncRNAs and proteins enriched in the pull‐downs. Ribosomal proteins except for S1 (RpsA) and proteins only co‐purified with tmRNA (except for SmpB) are omitted for clarity. Cbf1 is a recurring binding partner of the bait ncRNAs that also shows highly correlating sedimentation profiles in the gradient.
• C
  Heat map showing gradient profiles of Cbf1, csRNAs, and F41. Cbf1 co‐sediments with these sRNAs.

Source data are available online for this figure.