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. 2019 Dec 12;87(6):986–990. doi: 10.1038/s41390-019-0722-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Histological images of mouse epiphyseal cartilage before (a) and after (b) the growth plate is defined by the maturation of the secondary ossification center. Tissue sections from 3 days old (a) and 30 days old (b) mouse proximal tibiae are stained with Safranin O (red, cartilage) and Fast Green (green, bone and connective tissue).