Figure 5.

Time-dependent accumulation of covalently crosslinked Hb multimers in biological samples under hemolytic conditions. (a–d) Plasma and urine samples were collected from C57BL/6 mice at different time points (n = 5/group) following PHZ injection. (a, c) Representative western blots and (b, d) densitometric analysis of western blots are presented. The bar graph shows mean ± SD from 3 independent experiments. (e, f) CSF samples were obtained from preterm infants diagnosed with grade III IVH at different time intervals after the onset of IVH (n = 5/group). (e) Representative western blot is shown. Densitometric analysis was performed and percentages of Hb monomers, dimers, and tetramers as a percent of total Hb were calculated. p values were calculated using one-way ANOVA followed by Tukey's multiple comparison analysis. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.005, ^∗∗∗∗p < 0.001.