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. 2019 Dec 9;30(5):3030–3043. doi: 10.1093/cercor/bhz292

Figure 3.

Figure 3

Knockdown of SEPT7 in NPCs promotes differentiative cell divisions. (A) Dissociated cells from the E13.5 cortices of the Dcx-mRFP transgenic reporter mice were infected with lentiviruses for expression of shSept7-3UTR or scrambled control shRNA. shRNA-expressing (GFP positive) progenitor cells were imaged in live culture at intervals of 15 min for 48–72 h. Phase contrast and fluorescent images at indicated time were shown. Appearance of RFP expression in some daughter cells reflected the emerging neuronal fate in these nascent cells. Representative images of dividing cells in different division modes are shown (images are snap shots from supplementary movies). White arrows indicate mother and daughter progenitor cells. Red arrows indicate daughter cells taking a neuronal fate. Progenitor-progenitor (P-P), progenitor-neuron (P-N), or terminal neuron-neuron (N-N) division modes were scored. **P < 0.01 (Student’s t-test). Error bars represent SD. (B–G) Some representative lineage trees reconstructed from NPCs expressing scrambled control (B–D) shRNA or (E–G) shSept7-3UTR. IPC, intermediate progenitor cell.