Figure 4.

IUE-mediated knockout of SEPT7 causes neuronal differentiation of NPCs. (A) Cre expressing or control GFP-expressing plasmid was introduced into the cortex of Sept7^fl/fl embryos by IUE at E13.5, and the brains were analyzed at E16.5. Distributions of transfected cells in different radial regions of the cortex were scored. Scale bar represents 100 μm. ^** (in green and blue font) indicated P < 0.01 (Student’s t-test) with respect to the CP and VZ/SVZ distributions compared with control GFP. Error bars represent SD. (B) In the cortices of Sept7^fl/fl conditional knockout mice, Cre expressing cells (GFP⁺ cells) that have migrated into the CP were positive for NeuN, suggesting early differentiation into neurons due to Sept7 knockout. Arrows indicated examples of GFP⁺NeuN⁺ cells. Scale bar represents 100 μm. (C) Dissociated cells from the E13.5 cortices of Sept7^fl/fl mice were infected with lentiviruses for expression of GFP or Cre-2A-GFP. After culturing for two days post infection, cells were fixed and costained for KIF20A and KIF23/MKLP1. Percentage of telophase NPCs showing midbody (with noticeable MKLP1 staining) accumulation of KIF20A was scored. ^**P < 0.01 (Student’s t-test). Error bars represent SD.