Figure 6.

Inducible knockout of Sept7 in NPCs causes early cell cycle exit and precocious neuronal differentiation. (A) Tamoxifen was administered to Sept7 inducible knockout mice at E9.5 and E10.5 consecutively. EdU was labeled at E14.5 and embryonic brains were collected 24 h afterwards. R1, R2, and R3 indicated the EdU-labeled cell ribbons located progressively from superficial regions to the apical surface. EdU⁺Ki67⁻ cells were progenitor cells that had left the cell cycle. More EdU⁺Ki67⁻ cells in the total population of EdU⁺ cells were present in the cortices of the homozygous mutant cortices. Scale bar represents 100 μm. ^**P < 0.01 (Student’s t-test). Error bars represent SD. (B) EdU⁺Ki67⁻ cells that had migrated into the CP in the Nestin-CreERT2; Sept7^fl/fl homozygous mutant cortices were positive for NeuN, indicating that these cells had become neurons. Arrows indicated examples of EdU⁺Ki67⁻NeuN⁺ cells. Scale bar represents 100 μm.