KEY RESOURCES TABLE
REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Primary Antibodies | ||
Rabbit anti-GSX2 | Millipore | Cat# ABN162; RRID: AB_11203296 |
Chicken anti-GFP | Aves Labs | Cat# GFP-1020; RRID: AB_2307313 |
Goat anti-tdTomato | SICGEN | Cat# AB8181; RRID: AB_2722750 |
Rabbit anti-OLIG2 | Millipore | Cat# AB9610; RRID: AB_570666 |
Mouse anti-OLIG2 | Millipore | Cat# MABN50; RRID: AB_10807410 |
Goat anti-SP8 | Santa Cruz Biotechnology | Cat# sc-104661; RRID: AB_2194626 |
Rabbit anti-GFAP | Dako | Cat# Z0334; RRID: AB_10013382 |
Rabbit anti-S100 | Dako | Cat# Z0311; RRID: AB_10013383 |
Rabbit anti-ASCL1 | Cosmo Bio | Cat# SK-T01-003; RRID: AB_10709354 |
Guinea pig anti-DLX2 | Guo et al., 2019; Kuwajima et al., 2006 | |
Mouse anti-MKI67 | BD Pharmingen | Cat# 556003; RRID: AB_396287 |
Rabbit anti-SP9 | (Zhang et al., 2016) Available from authors | |
Rat anti-EOMES | Thermo Fisher | Cat# 12-4875-82; RRID: AB_1603275 |
Secondary antibodies | ||
Alexa Fluor®488 Donkey anti-Rabbit | Jackson ImmunoResearch | Cat# 711-546-152; RRID: AB_2340619 |
Cyanine Cy3 Donkey anti-Rabbit | Jackson ImmunoResearch | Cat# 711-166-152; RRID: AB_2313568 |
Alexa Fluor®488 Donkey anti-Goat | Jackson ImmunoResearch | Cat# 705-546-147; RRID: AB_2340430 |
Cyanine Cy3 Donkey anti-Goat | Jackson ImmunoResearch | Cat# 705-166-147; RRID: AB_2340413 |
Alexa Fluor®488 Donkey anti-Chicken | Jackson ImmunoResearch | Cat# 703-546-155; RRID: AB_2340376 |
Cyanine Cy3 Donkey anti-Guinea pig | Jackson ImmunoResearch | Cat# 706-166-148; RRID: AB_2340461 |
Alexa Fluor®488 Donkey anti-Rat | Jackson ImmunoResearch | Cat# 712-546-153; RRID: AB_2340686 |
Alexa Fluor®647 Donkey anti-Mouse | Jackson ImmunoResearch | Cat# 715-606-151; RRID: AB_2340866 |
Biological Samples | ||
Mouse cortex | This study | N/A |
Deposited Data | ||
Raw and processed data | This study | GEO: GSE140817 |
Experimental Models: Strains/Organisms | ||
Gsx2Flpo | This Study | N/A |
Rosa26-tdTomato-FRT | He et al., 2016 | N/A |
IS reporter | The Jackson Laboratory | Stock No. 028582 |
Emx1Cre/+ | The Jackson Laboratory | Stock No. 005628 |
SmoFlF | The Jackson Laboratory | Stock No. 004526 |
Rosa26SmoM2/+ | The Jackson Laboratory | Stock No. 005130 |
Gli2FlF | The Jackson Laboratory | Stock No. 007926 |
Gli3F/F | The Jackson Laboratory | Stock No. 008873 |
hGFAP-Cre | The Jackson Laboratory | Stock No. 004600 |
Recombinant DNA | ||
pCAG-Cre | Addgene | #13775 |
pCAG-GFP | Addgene | #11150 |
pCAG-ShhN-Ires-GFP | This Study | N/A |
pCAG-ShhN | This Study | N/A |
EdU | Life Technologies | E1087 |
Click-iT EdU Cell Proliferation Kit for Imaging | Invitrogen | C10337 |
Software and Algorithms | ||
Cell Ranger | 10X Genomics | https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome |
MACS | Xiaole Shirley Liu’s Lab | https://github.com/taoliu/MACS |
R | The R Project for Statistical Computing | https://www.r-project.org/ |
Seurat | Macosko et al., 2015 | https://satijalab.org/seurat/ |
Monocle2 | Qiu et al., 2017a, 2017b | https://cole-trapnell-lab.github.io/monocle-release/ |
GraphPad Prism 5.0 | GraphPad | https://www.graphpad.com |