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. Author manuscript; available in PMC: 2020 May 4.
Published in final edited form as: Cell Rep. 2020 Mar 31;30(13):4490–4504.e4. doi: 10.1016/j.celrep.2020.03.027

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Primary Antibodies
Rabbit anti-GSX2 Millipore Cat# ABN162; RRID: AB_11203296
Chicken anti-GFP Aves Labs Cat# GFP-1020; RRID: AB_2307313
Goat anti-tdTomato SICGEN Cat# AB8181; RRID: AB_2722750
Rabbit anti-OLIG2 Millipore Cat# AB9610; RRID: AB_570666
Mouse anti-OLIG2 Millipore Cat# MABN50; RRID: AB_10807410
Goat anti-SP8 Santa Cruz Biotechnology Cat# sc-104661; RRID: AB_2194626
Rabbit anti-GFAP Dako Cat# Z0334; RRID: AB_10013382
Rabbit anti-S100 Dako Cat# Z0311; RRID: AB_10013383
Rabbit anti-ASCL1 Cosmo Bio Cat# SK-T01-003; RRID: AB_10709354
Guinea pig anti-DLX2 Guo et al., 2019; Kuwajima et al., 2006
Mouse anti-MKI67 BD Pharmingen Cat# 556003; RRID: AB_396287
Rabbit anti-SP9 (Zhang et al., 2016) Available from authors
Rat anti-EOMES Thermo Fisher Cat# 12-4875-82; RRID: AB_1603275
Secondary antibodies
Alexa Fluor®488 Donkey anti-Rabbit Jackson ImmunoResearch Cat# 711-546-152; RRID: AB_2340619
Cyanine Cy3 Donkey anti-Rabbit Jackson ImmunoResearch Cat# 711-166-152; RRID: AB_2313568
Alexa Fluor®488 Donkey anti-Goat Jackson ImmunoResearch Cat# 705-546-147; RRID: AB_2340430
Cyanine Cy3 Donkey anti-Goat Jackson ImmunoResearch Cat# 705-166-147; RRID: AB_2340413
Alexa Fluor®488 Donkey anti-Chicken Jackson ImmunoResearch Cat# 703-546-155; RRID: AB_2340376
Cyanine Cy3 Donkey anti-Guinea pig Jackson ImmunoResearch Cat# 706-166-148; RRID: AB_2340461
Alexa Fluor®488 Donkey anti-Rat Jackson ImmunoResearch Cat# 712-546-153; RRID: AB_2340686
Alexa Fluor®647 Donkey anti-Mouse Jackson ImmunoResearch Cat# 715-606-151; RRID: AB_2340866
Biological Samples
Mouse cortex This study N/A
Deposited Data
Raw and processed data This study GEO: GSE140817
Experimental Models: Strains/Organisms
Gsx2Flpo This Study N/A
Rosa26-tdTomato-FRT He et al., 2016 N/A
IS reporter The Jackson Laboratory Stock No. 028582
Emx1Cre/+ The Jackson Laboratory Stock No. 005628
SmoFlF The Jackson Laboratory Stock No. 004526
Rosa26SmoM2/+ The Jackson Laboratory Stock No. 005130
Gli2FlF The Jackson Laboratory Stock No. 007926
Gli3F/F The Jackson Laboratory Stock No. 008873
hGFAP-Cre The Jackson Laboratory Stock No. 004600
Recombinant DNA
pCAG-Cre Addgene #13775
pCAG-GFP Addgene #11150
pCAG-ShhN-Ires-GFP This Study N/A
pCAG-ShhN This Study N/A
EdU Life Technologies E1087
Click-iT EdU Cell Proliferation Kit for Imaging Invitrogen C10337
Software and Algorithms
Cell Ranger 10X Genomics https://support.10xgenomics.com/single-cell-gene-expression/software/overview/welcome
MACS Xiaole Shirley Liu’s Lab https://github.com/taoliu/MACS
R The R Project for Statistical Computing https://www.r-project.org/
Seurat Macosko et al., 2015 https://satijalab.org/seurat/
Monocle2 Qiu et al., 2017a, 2017b https://cole-trapnell-lab.github.io/monocle-release/
GraphPad Prism 5.0 GraphPad https://www.graphpad.com