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. 2020 May 4;20:374. doi: 10.1186/s12885-020-06900-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Functional role of FAT4 on cell proliferation, colony formation, and invasive ability. a. Alamar blue assay displaying enhanced cell proliferation in FAT4 knocked MCAS and OVSAHO cells (p = 0.0001 and p = 0.0054 respectively) as compared to control. b. Colony formation assay in control and FAT4 siRNA treated MCAS, and OVSAHO cells. The assay shows a higher number of colonies in FAT4 siRNA treated cells. The cells were stained with crystal violet. c. Soft agar colony formation assay in control and FAT4 siRNA treated MCAS and OVSAHO cells. Anchorage-independent growth was significantly higher in cells treated with siFAT4 MCAS cells (p = 0.0055) and OVSAHO cells (p = 0.0302). d. Silencing FAT4 by siRNA promoted cell invasion in vitro in MCAS and OVSAHO cells (p = 0.0001 in MCAS and p = 0.003 in OVSAHO). MCAS and OVSAHO cells treated with scrambled siRNA were used as control. Data represent the mean and the standard deviation from at least three independent experiments performed in triplicates