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. 2020 May 4;20:49. doi: 10.1186/s12894-020-00617-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — miR-185 inhibited angiogenesis through the Nodal/ALK4 pathway. a The mRNA levels of ALK4 and VEGF were quantified by qRT-PCR in DU145 cells and LNCaP cells treated with Nodal or transfected with miR-185 mimics. b Protein levels of ALK4 and VEGF were assessed by Western blot in DU145 cells and LNCaP cells with the indicated treatments. c Representative photomicrographs of the colony formation assay and quantification of colony numbers in DU145 cells and LNCaP cells treated with Nodal or transfected with miR-185 mimics. d Cell viability of co-cultured HUVECs was evaluated by MTT assay and was determined as the percentage of the value relative to that of the control group. e The migration ability of co-cultured HUVECs was detected by transwell assay. f The tube forming ability of HUVECs was observed after the indicated treatment. The data represent one of three independent experiments. Error bars denote the mean ± SD. *P < 0.05, **P < 0.01