Table 2.
Antibodies used for immunostaining
| Antibody | Host species | Source | Catalog number | Dilutions |
|---|---|---|---|---|
| PDK1 | Rabbit | Epitomic | 3377–1 | 1:1000 |
| GAPDH | Rabbit | CST | 5174S | 1:5000 |
| AKT | Rabbit | CST | 9272S | 1:1000 |
| p-AKTThr308 | Rabbit | CST | 13038P | 1:500 |
| p-AKTSer473 | Rabbit | CST | 4060S | 1:1000 |
| GSK3β | Rabbit | CST | 9315S | 1:2000 |
| p-GSK3βSer9 | Rabbit | CST | 5558S | 1:1000 |
| PTEN | Rabbit | CST | 9188S | 1:1000 |
| PROX1 | Goat | R&D | AF2727 | 1:250 |
| Ascl1 | Rabbit | Santa Cruz | sc-28,688 | 1:250 |
| Olig2 | Rabbit | Millipore | AB9610 | 1:500 |
| PH3 | Rat | Abcam | ab10543 | 1:500 |
| BrdU | Rat | Abcam | ab6326 | 1:1000 |
| Ki67 | Rabbit | Abcam | ab16667 | 1:100 |
| Cleaved caspase-3 | Rabbit | Millipore | AB3623 | 1:1000 |
| HRP-linked anti-rabbit IgG | CST | 7074 | 1:5000 | |
| Alexa Fluor 488 goat anti-chicken | Molecular Probes | A11039 | 1:500 | |
| Alexa Fluor 488 donkey anti-rabbit | Molecular Probes | A11008 | 1:500 | |
| Alexa Fluor 633 goat anti-rabbit | Molecular Probes | A21071 | 1:500 | |
| Alexa Fluor 546 donkey anti-rabbit | Molecular Probes | A10040 | 1:500 | |
| Alexa Fluor 488 goat anti-rat | Molecular Probes | A11006 | 1:500 | |
| Alexa Fluor 546 goat anti-rat | Molecular Probes | A11081 | 1:500 | |
| Alexa Fluor 633 goat anti-rat | Molecular Probes | A21094 | 1:500 | |
| Alexa Fluor 546 rabbit anti-goat | Molecular Probes | A21085 | 1:500 | |