Table 3.

Physico-chemical characterization of E20c

Physico-chemical parameter	Treatment	Zone of inhibition (mm)
	Untreated control	16 ± 0.11
Temperature	60 °C (30 min)	16 ± 0.12
	60 °C (60 min)	14 ± 0.10
	80 °C (30 min)	14 ± 0.22
	80 °C (60 min)	14 ± 0.08
	100 °C (15 min)	14 ± 0.14
	100 °C (30 min)	13 ± 0.15
	100 °C (60 min)	13 ± 0.15
	121 °C (15 min)	–*
pH	2	10 ± 0.17
	4	16 ± 0.11
	6	16 ± 0.04
	8	13 ± 0.24
	10	–
Enzymes (1 mg/ml)	Proteinase K	–
	Pepsin	–
	Trypsin	–
	Lipase	16 ± 0.12

Zone of inhibition (mm) of E20c was determined by using agar gel diffusion assay against S. enterica. The results are the mean ± SD of three independent experiments preformed in triplicates

–, No zone of inhibition observed