Fig. 2.

Mettl1 methyltransf_4 domain is highly conserved evolutionally and liganded TR enhances Xenopus tropicalis Mettl1 promoter activity in vivo. a Amino acid alignment of Mettl1 from X. tropicalis, X. laevis, H. sapiens and M. musculus. The boxed region is the methyltransf_4 domain. Shaded amino acids indicate conserved residues. b Schematic representation of Mettl1 promoter and the first two exons. The putative TRE is shown as a white box. Gray box indicates the putative 5′ UTR of Mettl1 and black boxes indicate exons. The putative TRE is located at − 1128 bp from the predicted transcription start site of Mettl1 gene. c The Mettl1 promoter is activated by liganded TR in Xenopus oocytes. pGL4 was used as a negative control, and TRβ promoter construct was used as a positive control. The oocytes were injected with indicated mRNAs and reporter and harvested for luciferase assay. All data represent mean ± S.E.M. Significance value was ***P ≤ 0.005