Fig. 3.

Liganded TR activates the Mettl1 promoter through the putative TRE. a Schematic diagrams of the Mettl1 promoter construct with the putative TRE of Mettl1 (Mettl1 TRE) or a mutant TRE (Mettl1 mTRE). The TRE sequence is shown below the TRE with the mutated residues shown in red. The Mettl1 TRE and Mettl1 mTRE fragments were cloned into pGL4.10 luciferase reporter vector. b The Mettl1 wild type but not the mutant promoter construct is activated by liganded TR in Xenopus laevis oocytes. The oocytes were injected with indicated mRNAs and reporter and harvested for luciferase assay. All data represent mean ± S.E.M. Significance value was ***P ≤ 0.005. n.s. not significant