Fig. 4.

NSDHL knockdown suppresses total cholesterol level and promotes erlotinib response in MDA-MB-231 cell. a Total cholesterol levels measured in BT-20 and MDA-MB-231 cells transfected with NSDHL siRNA or control siRNA (20 nM); b Dose-response curve of erlotinib in BT-20 and MDA-MB-231 cells transfected with NSDHL siRNA or control siRNA (20 nM); c Representative western blot images of NSDHL, EGFR, and precursor and mature SREBP-1 and data of relative expression levels of NSDHL, EGFR, and precursor SREBP-1 in BT-20 and MDA-MB-231 cells transfected with NSDHL siRNA or control siRNA (20 nM). Data represent the mean ± standard deviation of three independent experiments. *p < 0.05, ***p < 0.001