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. 2019 Dec 16;30(5):3296–3312. doi: 10.1093/cercor/bhz310

Figure 2.

Figure 2

Dmrt5 is co-expressed with a subset of SP markers. (A, B) Coronal sections of the brain of E14.5 Lpar1-eGFP embryos immunostained for Dmrt5 (A) and of E16.5 embryos immunostained for Dmrt5 and Nurr1 (C). Areas outlined with dashed boxes are shown at higher magnification on the right (A’ and C’). White arrowheads in the insets indicate examples of co-labeled cells. Green and red arrowheads indicate Dmrt5−Lpar1-eGFP+ and Dmrt5+Lpar1-eGFP− cells, respectively, in A, and Dmrt5−Nurr1+ and Dmrt5+Nurr1− cells in C. Asterisks in right panels of A indicate Lpar1-GFP+Dmrt5+ co-labeled cells with neuronal morphology in CP. The proportion of co-labeled cells in the Lpar1-eGFP+ (37.2% ± 7.0) or Dmrt5+ populations (17.5% ± 4.4) at E14.5 (B; n = 12 sections from three brains) and in Nurr1+ (14.7% ± 5.6) or Dmrt5+ populations (35.1% ± 6.8) at E16.5 (D; n = 6 sections from two brains) are shown in the graphs. Data given are mean ± SD. Scale bars represent 100 μm in A and C and 25 μm in high-magnification boxes (A’ and C’).