. 2019 Dec 16;30(5):3296–3312. doi: 10.1093/cercor/bhz310

Table 1.

Summary of brain numbers (n) used for each figure and application

Figure and application		Genotype	Age	Preparation	Number
Fig. 1	Immunofluorescence	Wild type	E11.5, E12.5, E13.5, E14.5, E15.5, E16.5	Immersion fixed (PFA) and frozen	n = 3, 3, 2, 3, 3, 2
Fig. 1	Immunofluorescence	Dmrt5^−/−	E11.5, E12.5, E13.5, E14.5, E15.5, E16.5	Immersion fixed (PFA) and frozen	n = 3, 3, 2, 3, 3, 2
Fig. 2	Immunofluorescence	Lpar1-eGFP	E14.5	Fresh frozen	n = 3 (from 3 litters)
Fig. 2	Immunofluorescence	Wild type	E16.5	Immersion fixed (PFA) and frozen	n = 2 (from 2 litters)
Fig. 3	ISH	Wild type	E12.5; E18.5	Immersion fixed (PFA) and frozen	n = 3
	ISH	Dmrt5^−/−	E12.5; E18.5	Immersion fixed (PFA) and frozen	n = 3
	Immunofluorescence	Wild type	E11.5, E12.5, E13.5, E15.5		n = 3
	Immunofluorescence	Dmrt5^−/−	E11.5, E12.5, E13.5, E15.5		n = 3
Fig. 4	ISH	Wild type	E15.5; E18.5	Immersion fixed (PFA) and frozen	n = 3, 3
	ISH	Dmrt5^−/−	E15.5; E18.5		n = 3, 3
	Immunofluorescence	Wild type	E15.5		n = 2
	Immunofluorescence	Dmrt5^−/−	E15.5		n = 2
Fig. 5	Immunofluorescence	Wild type	E15.5, E18.5	Immersion fixed (PFA) and frozen	n = 3,3
		Dmrt5^−/−			n = 3,3
		Dmrt5^lox/lox; Emx1^CRE			n = 3,3
		Dmrt5^lox/lox; Nestin^CRE			n = 2,3
Fig. 6	Immunofluorescence	Wild type	E18.5 (BrdUE11.5; BrdUE12.5, BrdUE15.5)	Immersion fixed (PFA) and frozen	n = 3,3,2
Fig. 6	Immunofluorescence	Dmrt5^−/−	E18.5 (BrdUE11.5; BrdUE12.5, BrdUE15.5)	Immersion fixed (PFA) and frozen	n = 3,3,2
Fig. 7	Immunofluorescence	Wild type	E18.5 (BrdUE11.5; BrdUE12.5, BrdUE15.5)	Immersion fixed (PFA) and frozen	n = 3,3,3
Fig. 7	Immunofluorescence	Dmrt5^−/−	E18.5 (BrdUE11.5; BrdUE12.5, BrdUE15.5)	Immersion fixed (PFA) and frozen	n = 3,3,3
		Wild type	E18.5	Immersion fixed (PFA) and frozen	n = 4
		Dmrt5^−/−	E18.5		n = 4
Fig. 8	Immunofluorescence	Wild type	E15.5, E18.5		n = 2
		Dmrt5^−/−	E15.5, E18.5		n = 2
		Wild type	E18.5	Dissociated culture fixed (PFA)	n = 3 (from 3 litters)
		Dmrt5^−/−	E18.5	Dissociated culture fixed (PFA)	n = 3 (from 3 litters)
Fig. S1	Immunofluorescence	Wild type	E10.5, E11.5, E12.5	Immersion fixed (PFA) and frozen	n = 3 (from 3 litters)
Fig. S1	Immunofluorescence	Dmrt5^−/−	E10.5, E11.5, E12.5	Immersion fixed (PFA) and frozen	n = 3 (from 3 litters)
Fig. S2	Immunofluorescence	Wild type	E11.5, E12.5, E13.5, E14.5; E15.5	Immersion fixed (PFA) and frozen	n = 2, 3, 3, 3, 2
Fig. S2	Immunofluorescence	Dmrt5^−/−	E11.5, E12.5, E13.5, E14.5; E15.5	Immersion fixed (PFA) and frozen	n = 3, 3, 3, 3, 2
Fig. S3	Immunofluorescence	Wild type	E12.5	Immersion fixed (PFA) and frozen	n = 3
	Immunofluorescence	Dmrt5^−/−			n = 3
	Immunohistochemistry	Wild type			n = 3
	Immunohistochemistry	Dmrt5^−/−			n = 3
Fig. S4	Immunofluorescence	Wild type	E18.5	Immersion fixed (PFA) and frozen	n = 3
		Dmrt5^−/−			n = 3
		Dmrt5^lox/lox; Emx1^CRE			n = 3
		Dmrt5^lox/lox; Nestin^CRE			n = 2
Fig. S5	Immunofluorescence	Wild type	E18.5	Immersion fixed (PFA) and frozen	n = 2
	Immunofluorescence	Dmrt3^−/−;Dmrt5^−/−			n = 2
	ISH	Wild type			n = 2
	ISH	Dmrt3^−/−;Dmrt5^−/−			n = 2